plenti cmv puro dest plasmids (Addgene inc)

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Addgene inc plenti cmv puro dest plasmids
Plenti Cmv Puro Dest Plasmids, supplied by Addgene inc, used in various techniques. Bioz Stars score: 96/100, based on 332 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/plenti+cmv/pLenti+CMV+Puro+DEST+(w118-1)+(Plasmid+%2317452)/pm41921801-73-28-30
Average 96 stars, based on 332 article reviews

plenti cmv puro dest plasmids - by Bioz Stars, 2026-09

96/100 stars

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Construct:

Article Title: Parthenolide disrupts mitosis by inhibiting ZNF207/BUGZ-promoted kinetochore-microtubule attachment.
Article Snippet: The GFP-BUGZ construct was later subcloned into pLenti CMV/TO Puro DEST (Addgene #17293) destination vector by LR recombination according to manufacturer’s instructions, obtaining pLenti CMV/TO puro GFP- BUGZ plasmid. .. The GFP-BUGZ construct was later subcloned into pLenti CMV/TO Puro DEST (Addgene #17293) destination vector by LR recombination according to manufacturer’s instructions, obtaining pLenti CMV/TO puro GFP- BUGZ plasmid. ..

Article Title: Parthenolide disrupts mitosis by inhibiting ZNF207/BUGZ-promoted kinetochore-microtubule attachment
Article Snippet: BUGZ variants were then amplified by PCR using the primers 5′-GGTGGTGGTACCATGGGTCGCAAGAAGAAGAAG-3′ and 5′-GGTGGTGGATCCTCAGTAACGGCCACCTTG-3′ to introduce KpnI and BamHI sites and clone them into pENTR4-GFP (Addgene # 17396) vector for Gateway-compatible N-terminal GFP tagging to generate pENTR4-GFP-BUGZ and pENTR4-GFP-BUGZ C54A plasmids. .. The GFP-BUGZ construct was later subcloned into pLenti CMV/TO Puro DEST (Addgene #17293) destination vector by LR recombination according to manufacturer’s instructions, obtaining pLenti CMV/TO puro GFP-BUGZ plasmid. ..

Plasmid Preparation:

Article Title: Pertuzumab enhances the antitumor activity of T-DXd in HER2-positive gastric cancer cells
Article Snippet: 296 D ow nloaded from http://aacrjournals.org/m ct/article-pdf/doi/10.1158/1535-7163.M C T-24-0453/3670262/m ct-24-0453.pdf by guest on 16 N ovem ber 2025 14 297 Plasmid, virus production, and transduction 298 The pMDLg/pRRE (RRID:Addgene_12251, Addgene plasmid #12251), pRSV/REV 299 (RRID:Addgene_12253, Addgene plasmid #12253), and pMD2.G 300 (RRID:Addgene_12259, Addgene plasmid #12259) was a gifts from Professor Didier 301 Trono (11). .. Additionally, the pLenti CMV/TO V5-LUC Puro (RRID:Addgene_19785, 302 Addgene plasmid #19785) was a gift from Professors Eric Campeau and Paul Kaufman 303 (12). .. PEI (Sigma-Aldrich, St. Louis, MO, USA) was used to transfect plasmids into 293 304 T cells (Manassas, VA, USA) and polybrene (St Louis, MO, USA) was used to transfect 305 plasmids into NCI-N87 cells.

Article Title: CRISPR-Cas9 screening identifies ATOX1-driven cisplatin resistance mechanisms in liver cancer and evaluates targeted inhibitor efficacy
Article Snippet: .. For lentiviral packaging, pLKO.1-puro (Addgene plasmid #8453) and pLenti CMV/TO Puro empty (Addgene plasmid #17482) were obtained from Addgene (USA). ..

Article Title: CRISPR-Cas9 screening identifies ATOX1-driven cisplatin resistance mechanisms in liver cancer and evaluates targeted inhibitor efficacy.
Article Snippet: .. For lentiviral packaging, pLKO.1-puro (Addgene plasmid #8453) and pLenti CMV/TO Puro empty (Addgene plasmid #17482) were obtained from Addgene (USA). ..

Amplification:

Article Title: Oxidation of retromer complex controls mitochondrial translation.
Article Snippet: Junbing Zhang1,2,14 ✉, Md Yousuf Ali, Harrison Byron Chong, Pei-Chieh Tien, James Woods, Carolina Noble, Tristan Vornbäumen, Zehra Ordulu, Anthony P. Possemato, Stefan Harry, Jay Miguel Fonticella, Lina Fellah, Drew Harrison, Maolin Ge, Neha Khandelwal, Yingfei Huang, Maëva Chauvin, Anica Tamara Bischof, Grace Marie Hambelton, Magdy Farag Gohar, Siwen Zhang, MinGyu Choi, Sara Bouberhan, Esther Oliva, Mari Mino-Kenudson, Natalya N. Pavlova, Michael Lawrence, Justin F. Gainor, Sean A. Beausoleil, Nabeel Bardeesy, Raul Mostoslavsky, David Pépin, Christopher J. Ott, Brian Liau & Liron Bar-Peled1,7,10 ✉

Ligation:

Cloning:

Subcloning:

Article Title: Dynamic regulation of Sec24C by phosphorylation and O-GlcNAcylation during cell cycle progression
Article Snippet: .. Subcloning of N-terminally myc-6xHis-tagged WT Sec24C into pLenti CMV/TO Puro DEST (670–1) (Addgene, 17293) was previously described ( ) . ..

Stable Transfection:

Article Title:
Article Snippet: .. The following lentiviral destination vectors were used for stable cell line construction: pLenti CMV/TO zeo DEST with either human cHSF11 or CFP inserts (Addgene), pLenti6/V5 DEST Gateway with a tetracycline repressor insert (Invitrogen), pLenti CMV puro DEST (Invitrogen) with a DHFR.YFP fusion insert, and previously described DHFR.ATF6(1–373)- and XBP1s-encoding pLenti vectors.2 Cell Culture. .. Human T lymphocytes (CEM) were grown in RPMI-1640 medium (Corning) supplemented with 10% heat-inactivated fetal bovine serum (FBS; CellGro), 1% penicillin/streptomycin/glutamine (CellGro) at 37 °C with 5% CO2(g).

Review